The pCLEAN dual binary vector system for Agrobacterium-mediated plant transformation.
Identifieur interne : 000701 ( Main/Exploration ); précédent : 000700; suivant : 000702The pCLEAN dual binary vector system for Agrobacterium-mediated plant transformation.
Auteurs : Vera Thole [Royaume-Uni] ; Barbara Worland ; John W. Snape ; Philippe VainSource :
- Plant physiology [ 0032-0889 ] ; 2007.
Descripteurs français
- KwdFr :
- ADN bactérien (MeSH), Données de séquences moléculaires (MeSH), Génie génétique (MeSH), Génome végétal (MeSH), Oryza (génétique), Oryza (microbiologie), Plasmides (MeSH), Rhizobium (génétique), Tabac (génétique), Tabac (microbiologie), Transformation génétique (MeSH), Transgènes (MeSH), Vecteurs génétiques (MeSH).
- MESH :
- génétique : Oryza, Rhizobium, Tabac.
- microbiologie : Oryza, Tabac.
- ADN bactérien, Données de séquences moléculaires, Génie génétique, Génome végétal, Plasmides, Transformation génétique, Transgènes, Vecteurs génétiques.
English descriptors
- KwdEn :
- DNA, Bacterial (MeSH), Genetic Engineering (MeSH), Genetic Vectors (MeSH), Genome, Plant (MeSH), Molecular Sequence Data (MeSH), Oryza (genetics), Oryza (microbiology), Plasmids (MeSH), Rhizobium (genetics), Tobacco (genetics), Tobacco (microbiology), Transformation, Genetic (MeSH), Transgenes (MeSH).
- MESH :
- chemical : DNA, Bacterial.
- genetics : Oryza, Rhizobium, Tobacco.
- microbiology : Oryza, Tobacco.
- Genetic Engineering, Genetic Vectors, Genome, Plant, Molecular Sequence Data, Plasmids, Transformation, Genetic, Transgenes.
Abstract
The development of novel transformation vectors is essential to the improvement of plant transformation technologies. Here, we report the construction and testing of a new multifunctional dual binary vector system, pCLEAN, for Agrobacterium-mediated plant transformation. The pCLEAN vectors are based on the widely used pGreen/pSoup system and the pCLEAN-G/pCLEAN-S plasmids are fully compatible with the existing pGreen/pSoup vectors. A single Agrobacterium can harbor (1) pCLEAN-G and pSoup, (2) pGreen and pCLEAN-S, or (3) pCLEAN-G and pCLEAN-S vector combination. pCLEAN vectors have been designed to enable the delivery of multiple transgenes from distinct T-DNAs and/or vector backbone sequences while minimizing the insertion of superfluous DNA sequences into the plant nuclear genome as well as facilitating the production of marker-free plants. pCLEAN vectors contain a minimal T-DNA (102 nucleotides) consisting of direct border repeats surrounding a 52-nucleotide-long multiple cloning site, an optimized left-border sequence, a double left-border sequence, restriction sites outside the borders, and two independent T-DNAs. In addition, selectable and/or reporter genes have been inserted into the vector backbone sequence to allow either the counter-screening of backbone transfer or its exploitation for the production of marker-free plants. The efficiency of the different pCLEAN vectors has been assessed using transient and stable transformation assays in Nicotiana benthamiana and/or Oryza sativa.
DOI: 10.1104/pp.107.108563
PubMed: 17932303
PubMed Central: PMC2151721
Affiliations:
Links toward previous steps (curation, corpus...)
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Snape</name></author><author><name sortKey="Vain, Philippe" sort="Vain, Philippe" uniqKey="Vain P" first="Philippe" last="Vain">Philippe Vain</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Bacterial (MeSH)</term><term>Genetic Engineering (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Genome, Plant (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Oryza (genetics)</term><term>Oryza (microbiology)</term><term>Plasmids (MeSH)</term><term>Rhizobium (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (microbiology)</term><term>Transformation, Genetic (MeSH)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Génie génétique (MeSH)</term><term>Génome végétal (MeSH)</term><term>Oryza (génétique)</term><term>Oryza (microbiologie)</term><term>Plasmides (MeSH)</term><term>Rhizobium (génétique)</term><term>Tabac (génétique)</term><term>Tabac (microbiologie)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (MeSH)</term><term>Vecteurs génétiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryza</term><term>Rhizobium</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Oryza</term><term>Rhizobium</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Oryza</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Oryza</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Engineering</term><term>Genetic Vectors</term><term>Genome, Plant</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Transformation, Genetic</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN bactérien</term><term>Données de séquences moléculaires</term><term>Génie génétique</term><term>Génome végétal</term><term>Plasmides</term><term>Transformation génétique</term><term>Transgènes</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The development of novel transformation vectors is essential to the improvement of plant transformation technologies. Here, we report the construction and testing of a new multifunctional dual binary vector system, pCLEAN, for Agrobacterium-mediated plant transformation. The pCLEAN vectors are based on the widely used pGreen/pSoup system and the pCLEAN-G/pCLEAN-S plasmids are fully compatible with the existing pGreen/pSoup vectors. A single Agrobacterium can harbor (1) pCLEAN-G and pSoup, (2) pGreen and pCLEAN-S, or (3) pCLEAN-G and pCLEAN-S vector combination. pCLEAN vectors have been designed to enable the delivery of multiple transgenes from distinct T-DNAs and/or vector backbone sequences while minimizing the insertion of superfluous DNA sequences into the plant nuclear genome as well as facilitating the production of marker-free plants. pCLEAN vectors contain a minimal T-DNA (102 nucleotides) consisting of direct border repeats surrounding a 52-nucleotide-long multiple cloning site, an optimized left-border sequence, a double left-border sequence, restriction sites outside the borders, and two independent T-DNAs. In addition, selectable and/or reporter genes have been inserted into the vector backbone sequence to allow either the counter-screening of backbone transfer or its exploitation for the production of marker-free plants. The efficiency of the different pCLEAN vectors has been assessed using transient and stable transformation assays in Nicotiana benthamiana and/or Oryza sativa.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17932303</PMID><DateCompleted><Year>2008</Year><Month>03</Month><Day>31</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>145</Volume><Issue>4</Issue><PubDate><Year>2007</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>The pCLEAN dual binary vector system for Agrobacterium-mediated plant transformation.</ArticleTitle><Pagination><MedlinePgn>1211-9</MedlinePgn></Pagination><Abstract><AbstractText>The development of novel transformation vectors is essential to the improvement of plant transformation technologies. Here, we report the construction and testing of a new multifunctional dual binary vector system, pCLEAN, for Agrobacterium-mediated plant transformation. The pCLEAN vectors are based on the widely used pGreen/pSoup system and the pCLEAN-G/pCLEAN-S plasmids are fully compatible with the existing pGreen/pSoup vectors. A single Agrobacterium can harbor (1) pCLEAN-G and pSoup, (2) pGreen and pCLEAN-S, or (3) pCLEAN-G and pCLEAN-S vector combination. pCLEAN vectors have been designed to enable the delivery of multiple transgenes from distinct T-DNAs and/or vector backbone sequences while minimizing the insertion of superfluous DNA sequences into the plant nuclear genome as well as facilitating the production of marker-free plants. pCLEAN vectors contain a minimal T-DNA (102 nucleotides) consisting of direct border repeats surrounding a 52-nucleotide-long multiple cloning site, an optimized left-border sequence, a double left-border sequence, restriction sites outside the borders, and two independent T-DNAs. In addition, selectable and/or reporter genes have been inserted into the vector backbone sequence to allow either the counter-screening of backbone transfer or its exploitation for the production of marker-free plants. 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Snape</name><name sortKey="Vain, Philippe" sort="Vain, Philippe" uniqKey="Vain P" first="Philippe" last="Vain">Philippe Vain</name><name sortKey="Worland, Barbara" sort="Worland, Barbara" uniqKey="Worland B" first="Barbara" last="Worland">Barbara Worland</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Thole, Vera" sort="Thole, Vera" uniqKey="Thole V" first="Vera" last="Thole">Vera Thole</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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